Journal: Oncology Letters

Article Title: Antitumor activity of nimotuzumab in combination with cisplatin in lung cancer cell line A549 in vitro

doi: 10.3892/ol.2018.7923

Figure Lengend Snippet: Cyclin D1 expression in A549 cells in groups A-E following treatment for 48 h. Lanes: A, nimotuzumab; B, cisplatin; C, nimotuzumab followed by cisplatin; D, nimotuzumab and cisplatin simultaneously; and E, untreated control.

Article Snippet: Cell culture The A549 human lung adenocarcinoma epithelial cell line (supplied by the Central Laboratory of Qinhuangdao No. 1 People's Hospital) was cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Zhejiang Tianhang Biotechnology Co., Ltd., Huzhou, China), 1 U/ml penicillin and 1 mg/ml streptomycin at 37°C, with 5% CO 2 and 95% humidity.

Techniques: Expressing, Control

Journal: bioRxiv

Article Title: AngioPlate – Biofabrication of perfusable complex tissues in multi-well plates with 4D subtractive manufacturing

doi: 10.1101/2021.08.13.456244

Figure Lengend Snippet: a , Configuration of AngioPlate that allocates three inlet wells and two outlet wells for each terminal lung alveoli model. b , Design of the vascularized alveoli terminal with alveolar duct and sac and the resulting network perfused with FITC microparticles (1 µm, green) and TRITC microparticles (1 µm, red) for visualization. Scale bar, 1 mm. c , Vascularized alveoli terminal seeded with GFP-HUVECs (green) and alveolar epithelial cells (A549) on day 7. Scale bar, 1mm. d-e , Image and quantification of alveolar epithelium thickening over time. f-g , Fluorescent images of the cross-section of the vascularized alveolar sac stained for F-actin (red) and DAPI (blue). h , Histology cross-section of an vascularized terminal alveoli stained with Masson’s trichrome, E-Cadherin and CD-31. scale bar, 100 µm. i , A frame of a video that shows the physical expansion of the alveoli terminal actuated by a ventilator. j-k , Vector map and quantification that show the physical expansion of an alveolar sac at different levels of ventilation pressure. Statistical significance was determined using one-way ANOVA with the Holm-Sidak method. *P≤0.05 **P≤0.01 ***P ≤0.001

Article Snippet: Renal Proximal Tubule Epithelial Cells (RPTEC-TERT1, Everycyte, Cat# CHT-003-0002) and Lung Alveolar Epithelial Cells (A549, Cedarlane Labs, Cat# PTA-6231) were cultured as per manufacturer’s instructions.

Techniques: Staining, Plasmid Preparation